NASC

NASC ID: N849152

Name: WiscDsLox6B03

ABRC stock number: CS849152

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox6B03.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G07060.1-UTR3 and AT3G07070.1-P355 (at chr 3 pos 2237153 (C/2237153-2237231) on the TAIR10). The sequences' 1-79 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 1, C 6 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453460.

Wiscseq_DsLox6B03.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G59720.1 and AT1G59722.1 (chr 1 pos 21941826 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG810077.

Wiscseq_DsLox6B03.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G03160.1-UTR3 and AT1G03170.1-P355 (chr 1 pos 768402 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG810076.

Wiscseq_DsLox6B03.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G39460.1 (chr 4 pos 18359113 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG810078.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox6B03

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G03160 AT1G03170 AT1G59720 AT1G59722 AT3G07060 AT3G07070 AT4G39460